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In the first experiment, the TME of corn starch were determined by force-feeding 25 or 40 g of feed. Two experiments were conducted to investigate the feasibility of using corn starch as the basal diet to determine the ME of protein feedstuffs using the TME assay in Chinese Yellow chickens. Excellent agreement was obtained for RBV between the two assays, with rooster and chick values being similar and the ranking of the lipids being the same for the two assays. The RBV of SAB1 (22%), SAB2 (46%), and TW (76%), were lower (P < 0.001) than RCO, while PF (96%) and DDGSCO (97%) were not different than RCO. 2, BW increased linearly in proportion to the energy content of the lipid sources. The RBV of SAB1 and TW were lower (P < 0.001) than RCO, while PF and DDGSCO were not different than RCO. 1 a non-linear response was observed for SAB2 however, the RBV for SAB1 (22%), TW (72%), PF (96%), and DDGSCO (90%) were as expected. The RBV were calculated as the regression coefficient of the test lipid divided by that of RCO. The TMEn of diets or BW gain of chicks were regressed on supplemental lipid level. Chicks were limit-fed test diets from 10 to 20 d posthatch to maintain energy as the growth-limiting factor. 2, diets consisted of 0, 5, and 10% supplemental lipid in an amino-acid fortified corn-soybean meal diet.

1, roosters were tube-fed diets containing 0, 5, and 10% of supplemental lipid in ground corn. The same lipids were tested in both experiments and were refined corn oil (RCO), a 2:1 blend of stearic acid (C18:0) with RCO (SAB1), a 1:1 blend of C18:0 with RCO (SAB2), tallow (TW), poultry fat (PF), and corn oil from a corn ethanol plant (DDGSCO).

1) via the precision-fed rooster assay (PFRA) and broiler chicks via a growth assay (Exp.

Lipids that were expected to have widely varying ME content were evaluated using a slope-ratio approach in adult roosters (Exp. Our goal was to determine if relative bioavailability values (RBV) for fats and oils (lipids) determined in adult roosters are different than RBV determined with young broiler chickens.

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